Brine Shrimp Lethality Assay of Methanolic Extract of Orthosiphon Stamineus Benth. Leaves
The in vitro lethality of brine shrimp (BST), which is a simple tool for preliminary assessment of toxicity where one of the simplest biological responses to observe is lethality, since there is only one condition which are either dead or alive. The lethality of the methanol extract of Orthosiphon stamineus leaf from 10 different locations were subjected to brine shrimp after 24 h of exposure to the test solutions according to protocol reported by Meyer et al. (1982). The extracts were found to show reaction and dose dependent to toxicity on brine shrimp nauplii and the LC50 value of KBPP, TPPM, HLSM, SUMM,PPKM and DSJM were found to be 223.95 µg/ml, 252.04 µg/ml, 305.46 µg/ml, 323.47µg/ml, 291.00 µg/ml and 281.90 µg/ml respectively. The other four locations which are PTNS, SNNS, SKTM and CJPM were found to be 163.90 µg/ml, 130.06 µg/ml, 106.09µg/ml and 123.26 µg/ml respectively. This result revealed that these four locations gave positive results because about 50% of species presented positive results when LC50 <200µg/ml. A methanolic extract of this plant demonstrates moderate or low toxic activity against brine shrimp nauplii.
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