Total Flavonoid Content, Total Phenolic Content and Antioxidant Activities of Ethanol Extract for Piper sarmentosum Leaf from Ten Different Locations
The aim of this study is to determine the influence of geographical origin on the total flavonoid content (TFC), total phenolic content (TPC) and antioxidant activities of ethanol extract for Piper sarmentosum leaf from different origins. TFC and TPC assay used Aluminum Trichloride and Folin-Ciocalteu method, respectively. The antioxidant activity was measured using DPPH (1,1- diphenyl-2-picrylhidrazyl), β- carotene-linoleate and xanthine oxidase inhibition assay. The leaf extracts exhibited significant correlation between TPC and TFC with antioxidant activities. ANOVA analysis showed significant differences (p<0.05) between all sample in the TFC, TPC and antioxidant results. It suggests that different locations can influence the TFC, TPC and antioxidant activities of the plant extracts.
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